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世界上首臺(tái)顯微鏡誕生于16世紀(jì)，它的原理是使用光學(xué)系統(tǒng)將樣品放大以進(jìn)行分析。最早的顯微鏡利用自然光或聚光燈來(lái)照射樣品，觀察者一邊觀察一邊將看到的內(nèi)容徒手繪制出來(lái)。后來(lái)，各式各樣的顯微鏡陸續(xù)問(wèn)世，比如熒光顯微鏡、電子顯微鏡和掃描顯微鏡等等。
隨著攝影術(shù)的發(fā)明，1900年，科學(xué)家在利用顯微鏡的透鏡對(duì)樣品成像的同時(shí)，拍攝出世界上首張光學(xué)顯微照片。時(shí)至今日，經(jīng)過(guò)不斷發(fā)展，現(xiàn)在的顯微照相系統(tǒng)已經(jīng)能夠同時(shí)拍攝多張顯微照片，然后組合形成超高分辨率的清晰影像。通過(guò)不斷改進(jìn)光學(xué)鏡組、提高自動(dòng)化水平和位置反饋精度，未來(lái)的顯微照相技術(shù)必將迎來(lái)更大的進(jìn)步。
3DHISTECH公司位于匈牙利布達(dá)佩斯，它設(shè)計(jì)和生產(chǎn)了世界上運(yùn)行速度最快、通量最高的全自動(dòng)全視野數(shù)字切片掃描儀 — P1000。
P1000是一臺(tái)高精度掃描顯微鏡，大型病理學(xué)實(shí)驗(yàn)室可以用它采集醫(yī)學(xué)樣品切片的超高分辨率圖像，該設(shè)備一次性裝載切片后便可實(shí)現(xiàn)連續(xù)兩天的全自動(dòng)化工作，無(wú)需人工干預(yù)。這款新一代數(shù)字切片掃描儀基于3DHISTECH業(yè)已成熟的技術(shù)所開(kāi)發(fā)，是第一款集成了位置反饋光柵和磁編碼器的掃描儀，工作效率再創(chuàng)新高。
3DHISTECH公司首席技術(shù)官Viktor Varga詳細(xì)介紹道：“P1000一個(gè)工作輪次可以掃描1000張切片。它主要由兩部分組成：一臺(tái)數(shù)字切片掃描儀[顯微鏡]和一個(gè)高通量自動(dòng)切片載臺(tái)。兩大組件并行工作，提升了整個(gè)系統(tǒng)的效率。其顯微鏡有三個(gè)物鏡，可以進(jìn)行浸沒(méi)式及干式掃描。P1000可以滿足一般病理學(xué)或生物學(xué)實(shí)驗(yàn)室的所有掃描需求?！?br /> 顯微鏡的三根軸上都裝有搭配RTLC-S不銹鋼鋼帶柵尺的VIONiC數(shù)字增量式光柵系統(tǒng)，自動(dòng)切片載臺(tái)的拾放機(jī)械臂上則裝有LA11絕對(duì)式磁編碼器系統(tǒng)。這些光柵及磁編碼器不僅保證了設(shè)備的精度和重復(fù)性，而且提高了P1000的運(yùn)行速度，使其性能遠(yuǎn)超同類(lèi)產(chǎn)品。
挑戰(zhàn)

P1000的研發(fā)團(tuán)隊(duì)有一個(gè)明確的目標(biāo)：顯著提升大型病理學(xué)實(shí)驗(yàn)室的工作效率。他們發(fā)現(xiàn)，要實(shí)現(xiàn)這一目標(biāo)，掃描系統(tǒng)必須具有高通量，而且高度自動(dòng)化。
在掃描過(guò)程中，放有樣品的載物臺(tái)沿X軸和Y軸水平移動(dòng)，同時(shí)鏡筒垂直移動(dòng)以調(diào)節(jié)焦距。放大倍數(shù)越大，要求的精度就越高，這樣才能確保精確地拼接多張顯微照片，從而生成一幅超高分辨率圖像。
 

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